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Background:
This kit is designed for the detection of CREB-1 using the electrophoretic mobility shift assay (EMSA). Applications include screening for the presence of CREB-1 in various protein sources, evaluating the functional binding of CREB-1 recombinant proteins, analysis of different DNA-binding consensus sites and characterization of promoter elements.Contents:
The following are sufficient for 17 supershift reactions:3 x 40 µl PC-12 nuclear extract
90 µl CREB-1 rabbit pAb
12 µl ATF/CREB Oligo probe WT
40 µl ATF/CREB mutant Oligo
40 µl ATF/CREB Oligo WT
30 µL CREB-1 Peptide
240 µl Dilution Buffer A
240 µl Binding Buffer B-1
240 µl Binding Buffer C-1
360 µl Stabilizing Solution D
5 MicroSpin™ G-25 Columns
Application Notes:
When performing EMSA experiments individual optimization of extract and antigen quantities may be required in order to detect the protein of interest. For optimal results, we recommend the use of 10 µg of nuclear extract, 6 µl of antibody and 1X Tris-glycine electrophoresis buffer.Storage:
Upon receipt, the nuclear extract(s) must be stored at -80°C and the Microspin&trade columns should be stored at room temperature. All other components may be stored at either -20°C or -80°C. We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This avoids repeated freeze/thaw cycles.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
