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Antibodies

AML-1/Runx1 antibody (pAb)

Aliases: CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1
Catalog No: 39000 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:53 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for AML-1/Runx1 antibody (pAb)Application Notes


Validated Applications:
WB*: 1:500 - 1:1000

Published Applications:
ChIP
IP
WB
ICC/IF

See references for more information. Individual optimization may be required.

*For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for AML-1/Runx1 antibody (pAb)Immunogen

This AML-1/Runx1 antibody was raised against a synthetic peptide corresponding to amino acid residues 231-245 of human AML-1/Runx1.

for AML-1/Runx1 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for AML-1/Runx1 antibody (pAb)References

 
AML-1/Runx1 antibody (pAb) tested by Western blot.

AML-1/Runx1 antibody tested by Western blot.
Detection of AML-1/Runx1 by Western blot analysis. Positive detection of AML-1/Runx1 in nuclear extracts derived from Jurkat cells at 1:1,000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions.

for AML-1/Runx1 antibody (pAb)Background

AML-1 (acute myeloid leukemia 1, Runx1, CBFA2, PEBP2αB) is a transcription factor (activator) for various hematopoietic-specific genes (for example, GM-CSF, CSF1R and TCRb). AML-1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoters and enhancer elements. AML-1 is believed to be involved in the development of normal hematopoiesis and is commonly translocated in hematopoietic cancers.

for AML-1/Runx1 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for AML-1/Runx1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AML-1/Runx1 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AML-1/Runx1 antibody (pAb).

for AML-1/Runx1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AML-1/Runx1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AML-1/Runx1 antibody (pAb)
 

Data Thumbnails

AML-1/Runx1 antibody (pAb) tested by Western blot.

Western blot of AML-1/Runx1 pAb.
(Click image to enlarge and see details.)