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Antibodies

AP-2 antibody (pAb)

Aliases: TFAP2A, BOFS, AP2TF, TFAP2, AP-2alpha
Catalog No: 39001 Format: 100 µg $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:50 and 40 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated

for AP-2 antibody (pAb)Application Notes


Validated Applications:
WB*: 1:500 - 1:1,000

Published Applications:
ChIP
IHC
EMSA


See references for more information. Individual optimization may be required.

*For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for AP-2 antibody (pAb)Immunogen

This AP-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 120-134 of human AP-2.

for AP-2 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for AP-2 antibody (pAb)References

 
AP-2 antibody (pAb) tested by Western blot.

AP-2 pAb tested by Western blot.
Detection of AP-2 by Western blot analysis. Activator protein 2 (AP-2) can be detected in JEG-3 nuclear extract using AP-2 rabbit polyclonal antibody at a 1:1,000 dilution.

for AP-2 antibody (pAb)Background

AP-2 (activator protein 2 or AP2) transcription factors activate transcription and regulate gene expression during embryogenesis. There are four different isoforms of AP-2 (AP-2α, AP-2β, AP-2γ and AP-2δ). The family members share a common structure in which the N-terminal region is involved with transcriptional activation and the C-terminal region is involved in DNA binding.

for AP-2 antibody (pAb)Positive Control

Active Motif's JEG-3 nuclear extract (Catalog No. 36012) can be used as a positive control for AP-2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AP-2 antibody (pAb).

for AP-2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AP-2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AP-2 antibody (pAb)
 

Data Thumbnails

AP-2 antibody (pAb) tested by Western blot.

Western blot of AP-2 pAb.
(Click image to enlarge and see details.)