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Antibodies

c-Myc antibody (pAb) (65 kDa form)

Aliases: MRTL, MYCC, c-Myc, bHLHe39
Catalog No: 39012 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:65 kDa
Reactivity:Human, Monkey, Mouse

Applications

Western Blot Validated

for c-Myc antibody (pAb) (65 kDa form)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed overnight at 37°C. Individual optimization may be required.

for c-Myc antibody (pAb) (65 kDa form)Immunogen

This c-Myc antibody was raised against a synthetic peptide corresponding to amino acid residues 9-22 of human c-Myc; it recognizes a 65 kDa form of c-Myc.

for c-Myc antibody (pAb) (65 kDa form)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for c-Myc antibody (pAb) (65 kDa form)References

 
c-Myc antibody (pAb) (65 kDa form) tested by Western blot.

c-Myc antibody (65 kDa form) tested by Western blot.
Detection of c-Myc by Western blot analysis. A 65 kDa form of the transcription factor c-Myc is detected in nuclear extracts derived from 3T6 Swiss albino cells using c-Myc rabbit polyclonal antibody at a 1:1,000 dilution (lane 1). The signal is eliminated when the c-Myc immunizing peptide is added to the reaction mixture (lane 2).

for c-Myc antibody (pAb) (65 kDa form)Background

c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

for c-Myc antibody (pAb) (65 kDa form)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for c-Myc antibody (65 kDa form).

for c-Myc antibody (pAb) (65 kDa form)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for c-Myc antibody (pAb) (65 kDa form)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

c-Myc antibody (pAb) (65 kDa form)
 

Data Thumbnails

c-Myc antibody (pAb) (65 kDa form) tested by Western blot.

Western blot of c-Myc pAb (65 kDa form).
(Click image to enlarge and see details.)