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Antibodies

Sp1 antibody (pAb)

Aliases: PSG1, B1G1, PBG1, CD66f, PSBG1, PSG95, PSGGA, DHFRP2, PSBG-1, PSGIIA, FL-NCA-1/2, PS-beta-C/D, PS-beta-G-1
Catalog No: 39058 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:97 kDa
Reactivity:Human, Mouse, Rat

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Sp1 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
WB: 1:500 - 1:1,000 dilution

Published Applications:
ChIP-Seq
ChIP
WB
See references for more information. Individual optimization may be required.

For optimal results in Western blotting, primary antibody incubations should be performed at 37°C. Individual optimization may be required.

for Sp1 antibody (pAb)Immunogen

This Sp1 antibody was raised against a synthetic peptide corresponding to amino acid residues 520-534 of human Sp1.

for Sp1 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for Sp1 antibody (pAb)References

 
Sp1 antibody (pAb) tested by ChIP.

Sp1 antibody tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 20 µg of chromatin from B cell lymphoma Namalwa cells and 6 µl of Sp1 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Sp1 antibody (pAb) tested by Western blot.

Sp1 antibody tested by Western blot.
Detection of Sp1 by Western blot analysis. Sp1 was detected in nuclear extracts derived from K-562 cells using Sp1 rabbit polyclonal antibody at a 1:1,000 dilution.

for Sp1 antibody (pAb)Background

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.

for Sp1 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for Sp1 antibody.

for Sp1 antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Sp1 antibody (pAb).

for Sp1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Sp1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Sp1 antibody (pAb)
 

Data Thumbnails

Sp1 antibody (pAb) tested by ChIP.

ChIP of Sp1 pAb.
(Click image to enlarge and see details.)

Sp1 antibody (pAb) tested by Western blot.

Western blot of Sp1 pAb.
(Click image to enlarge and see details.)