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Antibodies

Lamin B1 antibody (pAb)

Aliases: LMNB1, LMN, ADLD, LMN2, LMNB
Catalog No: 39095 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:66-70 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for Lamin B1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:100 - 1:500 dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Lamin B1 antibody (pAb)Immunogen

This Lamin B1 antibody was raised against a full-length protein corresponding to human Lamin B1.

for Lamin B1 antibody (pAb)Buffer

Rabbit serum containing 0.1% sodium azide. Sodium azide is highly toxic.

for Lamin B1 antibody (pAb)References

 
Lamin B1 antibody (pAb) tested by Western blot.

Lamin B1 antibody tested by Western blot.
Lamin B1 detection by Western blot analysis. Lamin B1 is detected in HeLa nuclear extracts using Lamin B1 rabbit polyclonal antibody at a 1:100 (lane 1) and a 1:500 (lane 2) dilution.

for Lamin B1 antibody (pAb)Background

Lamin B1 (LMN, ADLD), along with the A and C Lamins are involved in maintaining the spatial organization of nuclear compartments. Depletion of Lamin B1 results in the disruption of RNA synthesis by RNA pol II, and eventually migration of chromatin to the nuclear periphery and dispersion of the nucleoli. The role of Lamin B1 in maintaining the nucleoskeleton may be to bind to nucleophosmin/B23.

for Lamin B1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Lamin B1 antibody.

for Lamin B1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Lamin B1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Lamin B1 antibody (pAb)
 

Data Thumbnails

Lamin B1 antibody (pAb) tested by Western blot.

Western blot of Lamin B1 pAb.
(Click image to enlarge and see details.)