| Isotype: | Rabbit IgG |
| Application: | Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western blot |
| Reactivity: | Human, Mouse |
|
| Purification: | None |
| Molecular Weight: | 90 kDa |
|
Background:
EZH2 is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein, and contains a SET domain that catalyzes the methylation of histone H3 at lysine 27. Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and SUZ12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. Deregulation of EZH2 is linked to a variety of cancers.
Immunogen:
This antibody was raised against recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.
Buffer:
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Application Notes:
This product has been tested by Western blotting (1:500 - 1:2,000).
Acid extract of HeLa cells (20 µg) probed with Catalog No. 39103 (1:1,000 dilution).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
YOUR CART
View Data Sheet
