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Histone H2A.Z antibody (pAb)
| Catalog No: 39113 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39114 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:1,000 - 1:5,000 | 2 - 10 µl per ChIP |
for Histone H2A.Z antibody (pAb) (H2AZ)Background
The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may serve to allow H2A.Z to function as an insulator of chromatin domains.
for Histone H2A.Z antibody (pAb) (H2AZ)Immunogen
This histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.
for Histone H2A.Z antibody (pAb) (H2AZ)Application Notes
This Histone H2A.Z antibody (H2AZ) has been validated for use in chromatin IP (2 - 10 µl per ChIP) and Western blotting (1:1,000 - 1:5,000 dilution); its specificity was verified by peptide competition. This antibody shows no reactivity toward recombinant histone H2A.
N.B. This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum free culture medium (NOT PBS!) prior to fixation.
for Histone H2A.Z antibody (pAb) (H2AZ)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H2A.Z antibody (pAb) (H2AZ)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2A.Z antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2A.Z antibody (pAb).
Histone H2A.Z antibody tested by Western blot.
Detection of H2A.Z by Western blot. The analysis was performed using Histone H2A.Z pAb at a 1:5,000 dilution.
Lane 1: 20 ng recombinant histone H2A.Z protein.
Lane 2: 5 µg HeLa acid extract.
Lane 3: 5 µg HeLa acid extract plus 1 µM immunizing peptide.
Histone H2A.Z antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H2A.Z pAb or 10 µl of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H2A.Z antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for histone H2A.Z. Recombinant proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:4,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: recombinant Histone H2A.
Lane 2: recombinant Histone H2A.Z.
Lane 3: recombinant Histone H4.
for Histone H2A.Z antibody (pAb) (H2AZ)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2A.Z antibody (pAb) (H2AZ)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
