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Histone H2AX phospho Ser139 antibody (pAb)
| Catalog No: 39117 | Format: 200 µg | $340 | Buy Now |
| Catalog No: 39118 | Format: 10 µg | $70 | Buy Now |
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| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:5,000 | Not tested. | 1:500 |
for Histone H2AX phospho Ser139 antibody (pAb)Background
Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.
for Histone H2AX phospho Ser139 antibody (pAb)Immunogen
This Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX.
for Histone H2AX phospho Ser139 antibody (pAb)Application Notes
This Histone H2AX phospho Ser139 antibody (H2AXS139Ph) has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution) and in immunofluorescence on HeLa cells with or without gamma irradiation to cause double-strand DNA breaks (1:500 dilution). Specificity for phospho-serine 139 was confirmed by dot blot with non-modified H2AX peptide or a peptide phosphorylated at serine 139.
for Histone H2AX phospho Ser139 antibody (pAb)Buffer
10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide.
for Histone H2AX phospho Ser139 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2AX phospho Ser139 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2AX phospho Ser139 antibody (pAb).
Histone H2AX phospho Ser139 antibody tested by immunofluorescence.
HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain™ Immunofluorescence Tools. The HeLa cells were blocked with MAXblock™ Blocking Medium and stained with Histone H2AX phospho Ser139 antibody.
Panel A: Untreated HeLa cells.
Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment.
Top images: Cells were stained with DAPI.
Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody.
Bottom images: Merge of both images above.
Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.
Histone H2AX phospho Ser139 antibody tested by immunofluorescence.
Etoposide-treated HeLa cells stained with Cat. No. 39117 (1:500 dilution, red) and counterstained with DAPI (blue).
Histone H2AX phospho Ser139 antibody tested by Western blot.
Western blot: Nuclear extract of U2OS cells (20 µg per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution).
Lane 1: untreated cells
Lane 2: cells treated with camptothecin
for Histone H2AX phospho Ser139 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2AX phospho Ser139 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
