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Antibodies

Histone H2A.XS139ph antibody (pAb)

Catalog No: 39117 Format: 200 µg $410 Buy Now
Catalog No: 39118 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:15 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Application Notes


Validated Applications:
ICC/IF: 1:500 dilution
WB: 1:1,000 - 1:5,000 dilution

Published Applications:
ICC/IF
See references for more information. Individual optimization may be required.

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Immunogen

This Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX.

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Buffer

10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide.

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)References

 
Histone H2A.XS139ph antibody (pAb) tested by immunofluorescence.

Histone H2AX phospho Ser139 antibody tested by immunofluorescence.
HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain™ Immunofluorescence Tools. The HeLa cells were blocked with MAXblock™ Blocking Medium and stained with Histone H2AX phospho Ser139 antibody.
Panel A: Untreated HeLa cells.
Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment.
Top images: Cells were stained with DAPI.
Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody.
Bottom images: Merge of both images above.
Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX.

Histone H2A.XS139ph antibody (pAb) tested by immunofluorescence.

Histone H2AX phospho Ser139 antibody tested by immunofluorescence.
Etoposide-treated HeLa cells stained with Cat. No. 39117 (1:500 dilution, red) and counterstained with DAPI (blue).

Histone H2A.XS139ph antibody (pAb) tested by Western blot.

Histone H2AX phospho Ser139 antibody tested by Western blot.
Western blot: Nuclear extract of U2OS cells (20 µg per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution).

Lane 1: untreated cells
Lane 2: cells treated with camptothecin

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Background

Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2AX phospho Ser139 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2A.XS139ph antibody (pAb).

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A.X phospho Ser139 antibody (pAb) (H2AX)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2A.XS139ph antibody (pAb)
 

Data Thumbnails

Histone H2A.XS139ph antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H2A.XS139ph pAb.
(Click image to enlarge and see details.)

Histone H2A.XS139ph antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H2A.XS139ph pAb.
(Click image to enlarge and see details.)

Histone H2A.XS139ph antibody (pAb) tested by Western blot.

Western blot of Histone H2A.XS139ph pAb.
(Click image to enlarge and see details.)