Histone H2B antibody (pAb)

for Histone H2B antibody (pAb)Background

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H2B antibody (pAb)Immunogen

This antibody was raised against a peptide including the C-terminal region of histone H2B.

for Histone H2B antibody (pAb)Application Notes

This Histone H2B antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and recognizes recombinant human H2B. Because this sequence is conserved from Drosophila to humans, a wide range of reactivity is expected.

for Histone H2B antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H2B antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2B antibody.

for Histone H2B antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H2B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.