Histone H3 acetyl antibody (pAb)

for Histone H3 acetyl antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation.

for Histone H3 acetyl antibody (pAb)Immunogen

This antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of histone H3.

for Histone H3 acetyl antibody (pAb)Application Notes

This Histone H3 acetyl antibody (H3Ac) has been validated for use in chromatin IP (10 µl per ChIP) and in Western blotting (1:500 - 1:5,000 dilution). Specificity for acetyl-lysines 9, 14, 18, 23 and 27 were confirmed by dot blot with non-modified histone H3 peptide or peptides acetylated at lysines 9, 14, 18, 23 or 27. Because these modifications are conserved from yeast to human, a wide range of reactivity is expected.

for Histone H3 acetyl antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H3 acetyl antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl antibody.

for Histone H3 acetyl antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 acetyl antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.