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Antibodies

Histone H3K4me2 antibody (pAb)

Catalog No: 39141 Format: 200 µl $420 Buy Now
Catalog No: 39142 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3 dimethyl Lys4 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
ChIP-Seq: 3 µl each
WB: 1:1,000 - 1:5,000 dilution

Published Applications:
ChIP
ChIP-Seq
IP
WB

See references for more information. Individual optimization may be required.

for Histone H3 dimethyl Lys4 antibody (pAb)Immunogen

This Histone H3 dimethyl Lys4 antibody was raised against a peptide including dimethyl-lysine 4 of histone H3.

for Histone H3 dimethyl Lys4 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39913) of this antibody that was purified by Protein A Chromatography is also available.

for Histone H3 dimethyl Lys4 antibody (pAb)References

 
Histone H3K4me2 antibody (pAb) tested by ChIP-Seq.

Histone H3 dimethyl Lys4 antibody tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) and chromatin from 500,000 primary mouse T cells. ChIP DNA was sequenced on the Illumina GA II and 25 million sequence tags were mapped to identify H3K4me2 binding sites. The image shows the expected H3K4me2 binding pattern with enrichment at promoters and at locations outside of promoters.

Histone H3K4me2 antibody (pAb) tested by ChIP.

Histone H3 dimethyl Lys4 pAb tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 15 µl of Histone H3 dimethyl Lys4 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.

Histone H3 dimethyl Lys4 antibody specificity tested by peptide array analysis.

Histone H3 dimethyl Lys4 antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 dimethyl Lys4 antibody was applied at a dilution of 1:10,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.
Array Data File

Histone H3K4me2 antibody (pAb) tested by Western blot.

Histone H3 dimethyl Lys4 antibody tested by Western blot.
HeLa acid extract (10 µg) was probed with Histone H3 dimethyl Lys4 antibody (1:5,000 dilution).

Histone H3K4me2 antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Lys4 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys4 pAb for dimethyl-lysine 4 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation (lysine 4, lysine 9, lysine 27) were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: Unmodified Lys4 peptide. Lane 2: Monomethyl Lys4 peptide. Lane 3: Dimethyl Lys4 peptide. Lane 4: Trimethyl Lys4 peptide. Lane 5: Monomethyl Lys9 peptide. Lane 6: Unmodified Lys9 peptide. Lane 7: Dimethyl Lys9 peptide. Lane 8: Trimethyl Lys9 peptide. Lane 9: Unmodified Lys27 peptide. Lane 10: Monomethyl Lys27 peptide. Lane 11: Dimethyl Lys27 peptide. Lane 12: Trimethyl Lys27 peptide.

for Histone H3 dimethyl Lys4 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

for Histone H3 dimethyl Lys4 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 dimethyl Lys4 antibody.

for Histone H3 dimethyl Lys4 antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me2 antibody (pAb).

for Histone H3 dimethyl Lys4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 dimethyl Lys4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K4me2 antibody (pAb)
 

Data Thumbnails

Histone H3K4me2 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3K4me2 pAb.
(Click image to enlarge and see details.)

Histone H3K4me2 antibody (pAb) tested by ChIP.

ChIP of Histone H3K4me2 pAb.
(Click image to enlarge and see details.)

Histone H3 dimethyl Lys4 antibody specificity tested by peptide array analysis.

Histone H3K4me2 antibody (pAb) tested by Western blot.

Western blot of Histone H3K4me2 pAb.
(Click image to enlarge and see details.)

Histone H3K4me2 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K4me2 pAb.
(Click image to enlarge and see details.)