Your Cart
Browse
Filter
Histone H3 phospho Ser28 antibody (pAb)
| Catalog No: 39149 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39150 | Format: 10 µl | $70 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:10,000 | — |
for Histone H3 phospho Ser28 antibody (pAb)Background
Histone H3 is one of five main histone proteins involved as core components of the nucleosome, the basic building block of chromatin. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase.
for Histone H3 phospho Ser28 antibody (pAb)Immunogen
This antibody was raised against a peptide including phospho-serine 28 of histone H3.
for Histone H3 phospho Ser28 antibody (pAb)Application Notes
This Histone H3 phospho Ser28 antibody (H3S28Ph) has been validated for use in Western blotting of cells arrested in mitosis (1:500 - 1:10,000). Specificity for phospho-serine 28 was confirmed by dot blot with non-modified histone H3 peptide or a peptide phosphorylated at serine 10 of Histone H3. This Histone H3 phospho Ser28 antibody detects a peptide containing phospho-serine 28 with 5 to 10-fold greater specificity than a peptide containing phospho-serine 10. Because this modification is conserved from yeast to human, a wide range of reactivity is expected.
for Histone H3 phospho Ser28 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 phospho Ser28 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Ser28 antibody.
Histone H3 phospho Ser28 pAb tested by Western blot.
HeLa acid extract (5 µg per lane) was probed with Histone H3 phospho Ser28 pAb (1:5,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.
Histone H3 phospho Ser28 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser28 pAb for phospho-Ser 10 of histone H3. Peptides corresponding to regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39149 at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: Peptide phosphorylated at serine 10.
Lane 2: Unmodified serine 10 peptide.
Lane 3: Peptide phosphorylated at serine 28.
Lane 4: Unmodified serine 28 peptide.
for Histone H3 phospho Ser28 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 phospho Ser28 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
