Histone H3, C-terminal antibody (pAb)

for Histone H3, C-terminal antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H3, C-terminal antibody (pAb)Immunogen

This histone H3 C-terminal antibody was raised against a C-terminal peptide of histone H3.

for Histone H3, C-terminal antibody (pAb)Application Notes

This Histone H3 C-terminal antibody has been validated for use in Western blotting (1:5,000 - 1:15,000 dilution) and in ChIP (5 - 15 µl per ChIP). Because this sequence is conserved from yeast to human, a wide range of reactivity is expected. This ability of this antibody to recognize histone H3 seems to be unaffected by any post-translational modifications to H3.

for Histone H3, C-terminal antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H3, C-terminal antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3, C-terminal antibody.

for Histone H3, C-terminal antibody (pAb)References

Baskind et al (2009) Plos One 4:e4750.

for Histone H3, C-terminal antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3, C-terminal antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.