Histone H4 acetyl Lys8 antibody (pAb)

for Histone H4 acetyl Lys8 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The chromatin-remodeling complex SWI/SNF is recruited to promoters through the interaction of the bromodomain of the protein Brg-1, belonging to the SWI/SNF complex, and CBP-acetylated histone H4 Lysine 8, leading to a chromatin remodeling.

for Histone H4 acetyl Lys8 antibody (pAb)Immunogen

This antibody was raised against a peptide including acetyl-lysine 8 of histone H4.

for Histone H4 acetyl Lys8 antibody (pAb)Application Notes

This Histone H4 acetyl Lys8 antibody (H4K8Ac) has been validated for use in Western blotting (1:500 - 1:4,000 dilution). Specificity for acetyl-lysine 8 was confirmed by peptide competition with a non-modified histone H4 peptide and peptides acetylated at lysines 5, 12 or 16. Serum is non-reactive with recombinant human histone H4. Because this modification is conserved from yeast to human, a wide range of reactivity is expected. Overnight incubation with this antibody is recommended for Western blotting.

for Histone H4 acetyl Lys8 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H4 acetyl Lys8 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys8 antibody.

for Histone H4 acetyl Lys8 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H4 acetyl Lys8 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.