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Histone H4 trimethyl Lys20 antibody (pAb)
| Catalog No: 39180 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39181 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:5,000 | 10 µl per ChIP | Published. See Application Notes below. |
for Histone H4 trimethyl Lys20 antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
for Histone H4 trimethyl Lys20 antibody (pAb)Immunogen
This histone H4 trimethyl Lys20 antibody was raised against peptide including trimethyl-Lys20 of human histone H4.
for Histone H4 trimethyl Lys20 antibody (pAb)Application Notes
This Histone H4 trimethyl Lys20 antibody (H4K20me) has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution), chromatin IP (10 µl per ChIP) and reported to work in immunofluorescence; its specificity was verified by peptide competition. See references for additional details.
for Histone H4 trimethyl Lys20 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H4 trimethyl Lys20 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 trimethyl Lys20 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 trimethyl Lys20 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4 trimethyl Lys20 antibody (pAb).
for Histone H4 trimethyl Lys20 antibody (pAb)References
This antibody is described and used in Sims, J.K. et al (2006) J Biol Chem 281: 12760-12766.
Histone H4 trimethyl Lys20 antibody tested by Western blot.
Detection of trimethylated Histone H4 by Western blot. The analysis was performed using HeLa acid extract and Histone H4 trimethyl Lys20 antibody at a 1:1,000 dilution.
Histone H4 trimethyl Lys20 antibody tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H4 trimethyl Lys20 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.
for Histone H4 trimethyl Lys20 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 trimethyl Lys20 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
