PP2A antibody (pAb)

RRID: AB_2793178
Aliases: PPP2R4, PR53, PTPA
Catalog No: 39192 Format: 200 µl $510 Buy
Catalog No: 39193 Format: 10 µl $125 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
36 kDa
Reactivity:
Human, Mouse, Rat, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 2 - 10 µl per ChIP
WB*: 1:250 - 1:2,000 dilution>

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This PP2A antibody was raised against a peptide corresponding to amino acids 288-303 of human PP2A.

Buffer

PBS containing 30% glycerol, 0.035% sodium azide and 1 mg/ml BSA. Sodium azide is highly toxic.

 

PP2A antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 µl of PP2A antibody or the equivalent amount of IgG as a negative control.

PP2A pAb tested by Western blot.
Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).

Background

PP2AProtein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PP2A antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PP2A antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting