| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 37 kDa |
|
| Western Blot |
Chromatin IP |
| 1 - 2 µg/ml |
Not tested |
Background for APE antibody (pAb):
APE (apurinic endonuclease or apyrimidinic endonuclease) is a multifunctional
DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (
AP endonucleases) are classified according to their site of incision.
Immunogen for APE antibody (pAb):
This antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 36-52 and 216-232 of human APE.
Application Notes for APE antibody (pAb):
This APE antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Active Motif's
HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APE antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
APE pAb tested by Western blot.
APE detection by Western blot. The analysis of APE was performed using HeLa whole-cell extract and APE pAb at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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