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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | — |
for APE antibody (pAb)Background
APE (apurinic endonuclease or apyrimidinic endonuclease) is a multifunctional DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (AP endonucleases) are classified according to their site of incision.
for APE antibody (pAb)Immunogen
This antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 36-52 and 216-232 of human APE.
for APE antibody (pAb)Application Notes
This APE antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for APE antibody (pAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for APE antibody (pAb)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APE antibody.
APE pAb tested by Western blot.
APE detection by Western blot. The analysis of APE was performed using HeLa whole-cell extract and APE pAb at a 2 µg/ml dilution.
for APE antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for APE antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
