Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

APE antibody (pAb)

Aliases: APEX1, APX, APE1, APEN, APEX, HAP1, REF1
Catalog No: 39200 Format: 100 µg $410 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:37 kDa
Reactivity:Human

Applications

Western Blot Validated

for APE antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for APE antibody (pAb)Immunogen

This APE antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 36-52 and 216-232 of human APE.

for APE antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for APE antibody (pAb)References

 
APE antibody (pAb) tested by Western blot.

APE pAb tested by Western blot.
APE detection by Western blot. The analysis of APE was performed using HeLa whole-cell extract and APE pAb at a 2 µg/ml dilution.

for APE antibody (pAb)Background

APE (apurinic endonuclease or apyrimidinic endonuclease) is a multifunctional DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (AP endonucleases) are classified according to their site of incision.

for APE antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APE antibody.

for APE antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for APE antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

APE antibody (pAb)
 

Data Thumbnails

APE antibody (pAb) tested by Western blot.

Western blot of APE pAb.
(Click image to enlarge and see details.)