| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 54 kDa |
|
| Western Blot |
Chromatin IP |
| 1:500 - 1:1,000 |
Not tested |
Background for APEXL2 antibody (pAb):
APEXL2 (APEX2, apurinic endonuclease-like 2 or apyrimidinic endonuclease-like 2) is a multifunctional
DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (
AP endonucleases) are classified according to their site of incision.
APEXL2 (APEX2) is a Class II AP endonuclease that cleaves the phosphodiester backbone 5′ to the AP site; it may also be present in the mitochondria.
Immunogen for APEXL2 antibody (pAb):
This antibody was raised against a synthetic peptide corresponding to human APEXL2.
Application Notes for APEXL2 antibody (pAb):
This APEXL2 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Active Motif's
HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APEXL2 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
APEXL2 pAb tested by Western blot.
APEXL2 detection by Western blot. The analysis of APEXL2 was performed using HeLa whole-cell extract and APEXL2 pAb at a 1:500 dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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