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Antibodies

INHAT-1/TAF-1α/TAF-1β antibody (pAb)

Aliases: TAF1A, SL1, RAFI48, TAFI48
Catalog No: 39202 Format: 200 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:37 kDa
Reactivity:Human, Mouse, Rat

Applications

Western Blot Validated Immunocytochemistry Validated

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Published Applications:
WB
ICC

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Immunogen

This INHAT-1/TAF-1a/TAF-1b antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 66-81 and 135-151 of human INHAT-1/TAF-1a/TAF-1β.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)References

 
INHAT-1/TAF-1α/TAF-1β antibody (pAb) tested by Western blot.

INHAT-1/TAF-1α/TAF-1β pAb tested by Western blot.
INHAT-1 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and INHAT-1/TAF-1α/TAF-1β pAb at a 2 µg/ml dilution.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Background

INHAT (also designated TAF-1α, TAF-1β and inhibitor of histone acetyltransferases complex) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for INHAT-1/TAF-1α/TAF-1β antibody.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

INHAT-1/TAF-1α/TAF-1β antibody (pAb)
 

Data Thumbnails

INHAT-1/TAF-1α/TAF-1β antibody (pAb) tested by Western blot.

Western blot of INHAT-1/TAF-1α/TAF-1β pAb.
(Click image to enlarge and see details.)