INHAT-1/TAF-1α/TAF-1β antibody (pAb)

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Background

INHAT (also designated TAF-1α, TAF-1β and inhibitor of histone acetyltransferases complex) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Immunogen

This INHAT-1/TAF-1a/TAF-1b antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 66-81 and 135-151 of human INHAT-1/TAF-1a/TAF-1β.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Application Notes

This INHAT-1/TAF-1a/TAF-1b antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for INHAT-1/TAF-1α/TAF-1β antibody.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for INHAT-1/TAF-1a/TAF-1b antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.