Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

CGBP antibody (pAb)

Catalog No: 39203 Format: 200 µl $340 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human, Mouse
Molecular Weight:88 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for CGBP antibody (pAb)Background

CGBP (CpG binding protein) is a transcriptional activator protein that binds unmethylated CpG dinucleotides and plays a role in regulating gene expression and embryonic development. CGBP exhibits a different distribution pattern than other heterochromatin-associated proteins. It seems to co-localize with acetylated histones, indicating that CGBP is associated with active chromatin.

for CGBP antibody (pAb)Immunogen

This CGBP antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 73-90 and 641-656 of human CGBP.

for CGBP antibody (pAb)Application Notes

This CGBP antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for CGBP antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for CGBP antibody (pAb)Positive Control

Active Motif's Human Spleen nuclear extract (Catalog No. 36046) can be used as a positive control for CGBP antibody.

 
CGBP pAb tested by Western blot.

CGBP pAb tested by Western blot.
Detection of CGBP by Western blot analysis. Western blot analysis using CGBP pAb at a 1:1,000 dilution against 15 µg/lane of human spleen lysate.

for CGBP antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CGBP antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

CGBP antibody (pAb)
 

Data Thumbnails

CGBP pAb tested by Western blot.

Western blot of CGBP pAb.
(Click image to enlarge and see details.)