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| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 2 - 4 µg/ml | 3rd-party validated* | 3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see "Application Notes" below for any recommendations. | ||
for DNMT1 antibody (mAb)Background
DNMT1 – DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).
DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
for DNMT1 antibody (mAb)Immunogen
This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.
for DNMT1 antibody (mAb)Application Notes
This DNMT1 antibody has been validated for use in Western blotting (2 - 4 µg/ml) and has been reported to work in chromatin IP, immunoprecipitation and immunohistochemistry. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for DNMT1 antibody (mAb)Buffer
PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
for DNMT1 antibody (mAb)Positive Control
Whole-cell extracts from mouse ES cells can be used as a positive control for DNMT1 antibody.
Chromatin IP Kits & Reagents
Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with DNMT1 antibody (mAb).
Bridging Antibody to Improve ChIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP and IP experiments that use DNMT1 antibody (mAb).
for DNMT1 antibody (mAb)References
This antibody was used in ChIP and in Re-ChIP assays as described in Schnekenburger, M. et. al. (2007) Mol Cell Biol. 27(20): 7089–7101.
DNMT1 antibody (mAb) tested by Western blot.
Detection of DNMT1 by Western blot. The analysis was performed using mouse ES whole-cell extract and DNMT1 antibody (mAb) at a 2 µg/ml dilution.
for DNMT1 antibody (mAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for DNMT1 antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
