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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 2 - 4 µg/ml | 5 µg per ChIP | 1 - 2 µg/ml | 1 - 2 µg/ml |
for DNMT3B antibody (mAb) (Clone 52A1018)Background
DNMT3B – DNA methyltransferase (or DNMT 3B) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3.
The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
for DNMT3B antibody (mAb) (Clone 52A1018)Immunogen
This antibody was raised against a full-length protein corresponding to mouse DNMT3B.
for DNMT3B antibody (mAb) (Clone 52A1018)Application Notes
This DNMT3B antibody has been validated for use in Western blotting (2 - 4 µg/ml), chromatin IP (5 µg per ChIP), immunofluorescence (1 - 2 µg/ml) and immunoprecipitation (1 - 2 µg/ml). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for DNMT3B antibody (mAb) (Clone 52A1018)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for DNMT3B antibody (mAb) (Clone 52A1018)Positive Control
Whole-cell extracts from 293 cells transfected with DNMT3B can be used as a positive control for DNMT3B antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with DNMT3B antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use DNMT3B antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with DNMT3B antibody (mAb).
DNMT3B mAb tested by Western blot.
Detection of DNMT3B by Western blot. The analysis was performed using DNMT3B mAb at a 2 µg/ml dilution and whole-cell extracts from 293 cells that had been transfected with plasmid expressing either mouse DNMT3A (lane A) or DNMT3B (lane B).
for DNMT3B antibody (mAb) (Clone 52A1018)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for DNMT3B antibody (mAb) (Clone 52A1018)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
