Histone H2A antibody (pAb)

for Histone H2A antibody (pAb)Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H2A antibody (pAb)Immunogen

This antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5 and 81-96 of human Histone H2A.

for Histone H2A antibody (pAb)Application Notes

This Histone H2A antibody has been validated for use in Western blotting (2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Histone H2A antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Histone H2A antibody (pAb)Positive Control

Whole-cell extracts from PBMCs can be used as a positive control for Histone H2A antibody.

for Histone H2A antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H2A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.