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Antibodies

Histone H2A antibody (pAb)

Catalog No: 39209 Format: 100 µg $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:14 kDa
Reactivity:Human

Applications

Western Blot Validated

for Histone H2A antibody (pAb)Application Notes


Validated Applications:
WB: 2 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Histone H2A antibody (pAb)Immunogen

This Histone H2A antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5 and 81-96 of human Histone H2A.

for Histone H2A antibody (pAb)Buffer

PBS containing 0.02% sodium azide and 0.05% BSA. Sodium azide is highly toxic.

for Histone H2A antibody (pAb)References

 
Histone H2A antibody (pAb) tested by Western blot.

Histone H2A pAb tested by Western blot.
Detection of Histone H2A by Western blot. The analysis was performed using PBMC whole-cell extract and Histone H2A pAb at a a 2 µg/ml dilution.

for Histone H2A antibody (pAb)Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H2A antibody (pAb)Positive Control

Whole-cell extracts from PBMCs can be used as a positive control for Histone H2A antibody.

for Histone H2A antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2A antibody (pAb)
 

Data Thumbnails

Histone H2A antibody (pAb) tested by Western blot.

Western blot of Histone H2A pAb.
(Click image to enlarge and see details.)