INHAT-2/pp32 antibody (pAb)

for INHAT-2/pp32 antibody (pAb)Background

INHAT (pp32) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-I beta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.

for INHAT-2/pp32 antibody (pAb)Immunogen

This INHAT-2/pp32 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 87-103 and 231-246 of human INHAT-2/pp32.

for INHAT-2/pp32 antibody (pAb)Application Notes

This INHAT-2/pp32 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for INHAT-2/pp32 antibody (pAb)Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

for INHAT-2/pp32 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for INHAT-2/pp32 antibody.

for INHAT-2/pp32 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for INHAT-2/pp32 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.