| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 83 kDa |
|
| Western Blot |
Chromatin IP |
| 2 - 3 µg/ml |
Not tested |
Background for MLH-1 antibody (mAb):
MLH-1 (
COCA2 or
HNPCC) heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative
DNA mismatch repair system (MMR).
DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.
MLH-1 (
COCA2 or
HNPCC) introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.
DNA methylation would prevent cleavage and therefore ensure that only the newly mutated DNA strand will be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.
MLH-1 is implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in the case of major DNA damages.
MLH-1 heterodimerizes with MLH-3 to form MutL gamma which plays a role in meiosis.
Immunogen for MLH-1 antibody (mAb):
This antibody was raised against a synthetic peptide corresponding to human MLH-1.
Application Notes for MLH-1 antibody (mAb):
This MLH-1 antibody has been validated for use in Western blotting (2 - 3 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Whole-cell extracts from Molt-4 cells can be used as positive control for MLH-1 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
MLH-1 mAb tested by Western blot.
The analysis was performed using Molt-4 whole-cell extract and MLH-1 mAb at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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