MLH-1 antibody (mAb)

for MLH-1 antibody (mAb)Background

MLH-1 (COCA2 or HNPCC) heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. MLH-1 (COCA2 or HNPCC) introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore ensure that only the newly mutated DNA strand will be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH-1 is implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in the case of major DNA damages. MLH-1 heterodimerizes with MLH-3 to form MutL gamma which plays a role in meiosis.

for MLH-1 antibody (mAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to human MLH-1.

for MLH-1 antibody (mAb)Application Notes

This MLH-1 antibody has been validated for use in Western blotting (2 - 3 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for MLH-1 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for MLH-1 antibody (mAb)Positive Control

Whole-cell extracts from Molt-4 cells can be used as positive control for MLH-1 antibody.

for MLH-1 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MLH-1 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.