Rad17 phospho Ser647 antibody (pAb)

for Rad17 phospho Ser647 antibody (pAb)Background

Rad17 Ser647 (Rad24, CCYC, R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

for Rad17 phospho Ser647 antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to mouse Rad17 phosphorylated at serine 647 .

for Rad17 phospho Ser647 antibody (pAb)Application Notes

This Rad17 phospho Ser647 antibody has been validated for use in Western blotting (0.1 - 1.0 µg/ml) and been reported to work in immunofluorescence. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Rad17 phospho Ser647 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Rad17 phospho Ser647 antibody (pAb)Positive Control

A recombinant Rad17 protein containing the phosphorylated Ser647 residue can be used as a positive control for Rad17 phospho Ser647 antibody.

for Rad17 phospho Ser647 antibody (pAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Rad17 phospho Ser647 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.