| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | IP, WB |
| Reactivity: | Human |
| Molecular Weight: | 15 kDa |
|
| Western Blot |
Chromatin IP |
Immunoprecipitation |
| 2 µg/ml |
Not tested |
3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see “Application Notes” below for any recommendations. |
Background for UBE2N antibody (pAb):
UBE2N (
UBC13,
BLU,
ubiquitin-conjugating enzyme E2N) heterodimer with UBE2V2 catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome.
UBE2N mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation.
UBE2N also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage and acts together with the E3 ligase SHPRH in the poly-ubiquitination of PCNA 'Lys-164' upon genotoxic stress, which is required for DNA repair.
Immunogen for UBE2N antibody (pAb):
This antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 2-19 and 131-148 of human UBE2N.
Application Notes for UBE2N antibody (pAb):
This UBE2N antibody has been validated for use in Western blotting (2 µg/ml), and reported to work in immunoprecipitation. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Positive Control:
Active Motif's
Human Heart nuclear extract (Catalog No. 36041) can be used as a positive control for UBE2N antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
UBE2N pAb tested by Western blot.
UBE2N detection by Western blot. The analysis was performed using human heart whole-cell extract and UBE2N pAb at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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