| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 20 kDa |
|
| Western Blot |
Chromatin IP |
| 1 - 3 µg/ml |
Not tested |
Background for UBE2V1a antibody (pAb):
UBE2N and its variants
UBE2V1α and β are members of the E2 ubiquitin-conjugating enzyme family, and the homologs of yeast UBC13. The
UBE2V1/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK (
IKKα,
IKKβ Clone 10AG2,
IKKβ Clone 10A9B6,
IKKγ) and does not seem to involve protein degradation by the proteasome.
UBE2V1 plays a role in the activation of NFκB (
NFκB p50,
NFκB p65) mediated by IL-1β, TNF, TRAF6 and
TRAF2.
Immunogen for UBE2V1a antibody (pAb):
This UBE2V1a antibody was raised against a peptide corresponding to amino acid residues 31-49 of human UBE2V1a.
Application Notes for UBE2V1a antibody (pAb):
This UBE2V1a antibody has been validated for use in Western blotting (1 - 3 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Buffer:
PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Positive Control:
Active Motif's
HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for UBE2V1α antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
UBE2V1α pAb tested by Western blot.
Detection of UBE2V1α by Western blot. The analysis was performed using HeLa whole-cell extract and UBE2V1α pAb at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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