Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

Dimethyl-Arginine, asymmetric antibody (pAb)

Catalog No: 39231 Format: 200 µl $340 Buy Now
Catalog No: 39232 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:Serum
Purification:None
Appl.:IF, WB
Reactivity:Other (Not Species Specific)

Western Blot Chromatin IP Immunofluorescence
1:500 - 1:2,000 Not tested. 1:250 - 1:500

for Dimethyl-Arginine, asymmetric antibody (pAb)Background

Dimethyl-Arginine, asymmetric (or Asymmetric dimethylarginine, ADMA) interferes with the production of nitric oxide (NO). Arginine methylation is an important post-translational modification catalyzed by the PRMT/CARM family of methyltransferases.

for Dimethyl-Arginine, asymmetric antibody (pAb)Immunogen

This Dimethyl-Arginine, asymmetric antibody was raised against peptide containing dimethyl-arginine (asymmetric).

for Dimethyl-Arginine, asymmetric antibody (pAb)Application Notes

This Dimethyl-Arginine, asymmetric antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:250 - 1:500 dilution).

for Dimethyl-Arginine, asymmetric antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Dimethyl-Arginine, asymmetric antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Dimethyl-Arginine, asymmetric antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Dimethyl-Arginine, asymmetric antibody (pAb).

 
Dimethyl-Arginine, asymmetric pAb tested by Western blot.

Dimethyl-Arginine, asymmetric pAb tested by Western blot.
Detection of Dimethyl-Arginine by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and Dimethyl-Arginine, asymmetric pAb at a 1:1,000 dilution.

Dimethyl-Arginine, asymmetric pAb tested by immunofluorescence.

Dimethyl-Arginine, asymmetric pAb tested by immunofluorescence.
Staining of HeLa cells with Dimethyl-Arginine, asymmetric pAb (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

for Dimethyl-Arginine, asymmetric antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Dimethyl-Arginine, asymmetric antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Dimethyl-Arginine, asymmetric antibody (pAb)
 

Data Thumbnails

Dimethyl-Arginine, asymmetric pAb tested by Western blot.

Western blot of Dimethyl-Arginine, asymmetric pAb.
(Click image to enlarge and see details.)

Dimethyl-Arginine, asymmetric pAb tested by immunofluorescence.

Immunofluorescence stain of Dimethyl-Arginine, asymmetric pAb.
(Click image to enlarge and see details.)