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RNA pol II CTD phospho Ser5 antibody (pAb)
| Catalog No: 39233 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39234 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | 10 µl per ChIP | 1:250 - 1:1,000 |
for RNA pol II CTD phospho Ser5 antibody (pAb)Background
RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
for RNA pol II CTD phospho Ser5 antibody (pAb)Immunogen
This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.
for RNA pol II CTD phospho Ser5 antibody (pAb)Application Notes
This RNA pol II CTD phospho Ser5 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution), chromatin immunoprecipitation (10 µl per ChIP) and immunofluorescence (1:250 - 1:1,000 dilution).
for RNA pol II CTD phospho Ser5 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for RNA pol II CTD phospho Ser5 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA pol II CTD phospho Ser5 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA pol II CTD phospho Ser5 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RNA pol II CTD phospho Ser5 antibody (pAb).
RNA pol II CTD phospho Ser5 antibody tested by immunofluorescence.
Top image: HeLa cells stained with RNA pol II CTD phospho Ser5 antibody at a 1:1,000 dilution. Middle: Same cells stained with DAPI. Bottom: Merge of both images.
RNA pol II CTD phospho Ser5 antibody tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with RNA pol II CTD phospho Ser5 antibody at a 1:2,000 dilution.
RNA pol II CTD phospho Ser5 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of RNA pol II CTD phospho Ser5 antibody for phospho-Ser5 of the RNA Pol II C-terminal domain heptad repeat. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. Decreasing amounts of peptide were spotted in each row.
Lane 1: Peptide phosphorylated at CTD repeat serine 2.
Lane 2: Unmodified CTD repeat serine 2 peptide.
Lane 3: Peptide phosphorylated at CTD repeat serine 5.
Lane 4: Unmodified CTD repeat serine 5 peptide.
RNA pol II CTD phospho Ser5 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of RNA pol II CTD phospho Ser5 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for RNA pol II CTD phospho Ser5 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for RNA pol II CTD phospho Ser5 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
