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Histone H2A antibody (pAb)
| Catalog No: 39235 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39236 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:2,000 - 1:5,000 | 2 - 5 µl per ChIP |
for Histone H2A antibody (pAb)Background
Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.
Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
for Histone H2A antibody (pAb)Immunogen
This histone H2A antibody was raised against recombinant yeast histone H2A.
for Histone H2A antibody (pAb)Application Notes
This yeast histone H2A antibody has been validated for use in Western blotting (1:2,000 - 1:5,000 dilution) and chromatin IP (2 - 5 µl per ChIP).
for Histone H2A antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H2A antibody (pAb)Positive Control
Whole-cell extracts and acid extracts from yeast can be used as a positive control for Histone H2A antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2A antibody (pAb).
Histone H2A anbtibody (pAb) tested by Western blot.
Detection of Histone H2A by Western blot. The analysis was performed using S. cerevisiae acid extract probed with Histone H2A antibody at a 1:5,000 dilution.
for Histone H2A antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2A antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
