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Antibodies

CARM1 antibody (pAb)

Aliases: Coactivator Associated Arginine Methyltransferase 1, Protein Arginine N-Methyltransferase 4 (PRMT4)
Catalog No: 39251 Format: 200 µl $410 Buy Now
Catalog No: 39252 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

for CARM1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:

ChIP
IP
IHC
WB

for CARM1 antibody (pAb)Immunogen

This CARM1 antibody was raised against a peptide corresponding to the C-terminus of mouse CARM1.

for CARM1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CARM1 antibody (pAb)References

 
CARM1 antibody (pAb) tested by Western blot.

CARM1 pAb tested by Western blot.
Whole-cell extract of HEK293 cells (10 µg) probed with CARM1 pAb at a dilution of 1:500.

for CARM1 antibody (pAb)Background

CARM1 (Coactivator-associated arginine methyltransferase 1 or CARM 1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. CARM1 methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

for CARM1 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CARM1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CARM1 antibody (pAb).

for CARM1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CARM1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CARM1 antibody (pAb)
 

Data Thumbnails

CARM1 antibody (pAb) tested by Western blot.

Western blot of CARM1 pAb.
(Click image to enlarge and see details.)