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Antibodies

JMJD2F antibody (pAb)

Catalog No: 39257 Format: 200 µl $340 Buy Now
Catalog No: 39258 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:WB
Reactivity:Human
Molecular Weight:90 kDa

Applications Table

Western Blot Chromatin IP
1:400 - 1:1,000

for JMJD2F antibody (pAb)Background

JMJD2F is a member of the JMJD2 family of Histone H3 trimethyl Lys9 demethylase enzymes. JMJD2F contains both a JmjN and a JmjC domain.

for JMJD2F antibody (pAb)Immunogen

This JMJD2F antibody was raised against a peptide corresponding to the C-terminus of human JMJD2F.

for JMJD2F antibody (pAb)Application Notes

This JMJD2F antibody has been validated for use in Western blotting (1:400 - 1:1,000 dilution).

for JMJD2F antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for JMJD2F antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JMJD2F antibody.

 
JMJD2F antibody (pAb) tested by Western blot.

JMJD2F pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with JMJD2F pAb at a dilution of 1:1,000.

for JMJD2F antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for JMJD2F antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting

Technical Data Sheet

JMJD2F antibody (pAb)
 

Data Thumbnails

JMJD2F antibody (pAb) tested by Western blot.

Western blot of JMJD2F pAb.
(Click image to enlarge and see details).