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Antibodies

INCENP antibody (mAb)

Catalog No: 39259 Format: 200 µg $340 Buy Now
Catalog No: 39260 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein A Chromatography
Applications:IF
Reactivity:Human, Mouse, Other (Wide Range)
Molecular Weight:140 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
1:500 - 1:1,000

for INCENP antibody (mAb)Background

INCENP (Inner Centromere Protein) is part of the chromosomal passenger complex that associates with chromosomes and regulates mitosis and meiosis. INCENP forms a scaffold holding the CP complex together and plays a role in the regulation of Aurora B and Aurora C kinase activity.

for INCENP antibody (mAb)Immunogen

This antibody was raised against a recombinant protein corresponding to amino acid residues 231-398 of human INCENP.

for INCENP antibody (mAb)Application Notes

This INCENP antibody has been validated for use in immunofluorescence (1:500 - 1:1,000 dilution).

for INCENP antibody (mAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for INCENP antibody (mAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for INCENP antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with INCENP antibody (mAb).

 
INCENP mAb tested by immunofluorescence.

INCENP mAb tested by immunofluorescence.
Top left: HeLa cells stained with INCENP monoclonal antibody at a 1:1,000 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

for INCENP antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for INCENP antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

INCENP antibody (mAb)
 

Data Thumbnails

INCENP mAb tested by immunofluorescence.

Immunofluorescence stain of INCENP mAb.
(Click image to enlarge and see details.)