Histone H4 antibody (pAb)

for Histone H4 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H4 antibody (pAb)Immunogen

This antibody was raised against full-length recombinant Xenopus histone H4.

for Histone H4 antibody (pAb)Application Notes

This Histone H4 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution). It is not recommended for immunofluorescence. Recognition of histone H4 by this antibody is slightly compromised by acetylation.

for Histone H4 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H4 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 antibody.

for Histone H4 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.