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Antibodies

Histone H3K56me1 antibody (pAb)

Catalog No: 39273 Format: 200 µl $410 Buy Now
Catalog No: 39274 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for Histone H3 monomethyl Lys56 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

for Histone H3 monomethyl Lys56 antibody (pAb)Immunogen

This Histone H3 monomethyl Lys56 antibody was raised against a peptide including monomethyl-lysine 56 of human histone H3.

for Histone H3 monomethyl Lys56 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 monomethyl Lys56 antibody (pAb)References

 
Histone H3K56me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Lys56 pAb tested by Western blot.
HeLa acid extract (10 µg) was probed with Histone H3 monomethyl Lys56 pAb at a dilution of 1:5,000.

Histone H3K56me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Lys56 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys56 pAb for monomethyl Lys56 histone H3. Methylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Unmodified lysine 56 peptide.
    Lane 2: Monomethyl lysine 56 peptide.
    Lane 3: Dimethyl lysine 56 peptide.
    Lane 4: Trimethyl lysine 56 peptide.

No detection of peptides (unmodified, mono-, di-, or trimethylated) corresponding to lysine 4, lysine 9 or lysine 27 was observed with this antibody.

for Histone H3 monomethyl Lys56 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 56 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs).

for Histone H3 monomethyl Lys56 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys56 antibody.

for Histone H3 monomethyl Lys56 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 monomethyl Lys56 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K56me1 antibody (pAb)
 

Data Thumbnails

Histone H3K56me1 antibody (pAb) tested by Western blot.

Western blot of Histone H3K56me1 pAb.
(Click image to enlarge and see details.)

Histone H3K56me1 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K56me1 pAb.
(Click image to enlarge and see details.)