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Antibodies

Histone H3K56ac antibody (pAb)

Catalog No: 39281 Format: 100 µl $420 Buy Now
Catalog No: 39282 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Budding Yeast, Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3 acetyl Lys56 antibody (pAb)Application Notes


Validated Applications:
ChIP: 3 - 5 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:2,500 - 1:5,000 dilution

Published Applications:
ChIP-Seq
ChIP
WB
See references for more information. Individual optimization may be required.

for Histone H3 acetyl Lys56 antibody (pAb)Immunogen

This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.

for Histone H3 acetyl Lys56 antibody (pAb)Buffer

Purified rabbit IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 acetyl Lys56 antibody (pAb)References

 
Histone H3K56ac antibody (pAb) tested by ChIP-chip.

Histone H3K56ac antibody (pAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million HeLa cells and 5 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows H3K56Ac binding across a 650,000 bp region on chromosome 17. The image on the right shows H3K56Ac binding at the KLF5 gene.

Histone H3K56ac antibody (pAb) tested by ChIP.

Histone H3 acetyl Lys56 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 µl of Histone H3 acetyl Lys56 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3K56ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys56 antibody tested by Western blot.
HeLa acid extract (10 µg/lane) was probed with Histone H3 acetyl Lys56 polyclonal antibody (1:1,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.

Histone H3K56ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys56 antibody tested by Western blot.
Acid extract of yeast cells probed with Histone H3 acetyl Lys56 pAb (1:1,400 dilution).

Histone H3K56ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys56 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: H3 acetyl-Lys4 peptide. Lane 2: H3 acetyl-Lys9 peptide. Lane 3: H3 acetyl-Lys14 peptide. Lane 4: H3 acetyl-Lys18 peptide.. Lane 5: unmodified H3 peptide aa 13-22. Lane 6: H3 acetyl-Lys23 peptide. Lane 7: H3 acetyl-Lys27 peptide. Lane 8: H3 acetyl-Lys36 peptide. Lane 9: H3 acetyl-Lys37 peptide. Lane 10: yeast H3 acetyl-Lys56 peptide. Lane 11: unmodified yeast H3 peptide aa 50-59. Lane 12: human H3 acetyl-Lys56 peptide.

for Histone H3 acetyl Lys56 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

for Histone H3 acetyl Lys56 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys56 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K56ac antibody (pAb).

for Histone H3 acetyl Lys56 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys56 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K56ac antibody (pAb)
 

Data Thumbnails

Histone H3K56ac antibody (pAb) tested by ChIP-chip.

ChIP-chip of Histone H3K56ac pAb.
(Click image to enlarge and see details.)

Histone H3K56ac antibody (pAb) tested by ChIP.

ChIP of Histone H3K56ac pAb.
(Click image to enlarge and see details.)

Histone H3K56ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K56ac pAb.
(Click image to enlarge and see details.)

Histone H3K56ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K56ac pAb.
(Click image to enlarge and see details.)

Histone H3K56ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K56ac pAb.
(Click image to enlarge and see details.)