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Lamin A/C antibody (mAb)
| Clone: 3A6-4C11 |
| Catalog No: 39287 | Format: 200 µg | $340 | Buy Now |
| Catalog No: 39288 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 0.5 - 2 µg/ml | — | 5 - 10 µg/ml | 2 - 5 µg per IP |
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Background
Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Immunogen
This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Application Notes
This Lamin A/C antibody has been validated for use in Western blotting (0.5 - 2.0 µg/ml dilution), immunofluorescence (5 - 10 µg/ml dilution) and immunoprecipitation (2 - 5 µg per IP). Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer may help detection by Western blot.
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Buffer
Cell culture supernatant containing mouse IgG, 30% glycerol, 0.035% sodium azide.
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Lamin A/C antibody (Clone 3A6-4C11).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Lamin A/C antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Lamin A/C antibody (mAb).
Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot.
Whole-cell extract (20 µg) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 0.5 µg/ml.
Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence.
Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11) (2 µg/ml dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Lamin A/C antibody (mAb) (Clone 3A6-4C11)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
