Your Cart
Browse
Filter
Aiolos antibody (pAb)
| Catalog No: 39293 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39294 | Format: 10 µl | $70 | Buy Now |
|
|
| Western Blot | Chromatin IP | Immunofluorescence | Immunoprecipitation |
|---|---|---|---|
| 1:500 - 1:1,000 | Not tested. | 3rd-party validated* | 3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see "Application Notes" below for any recommendations. | |||
for Aiolos antibody (pAb)Background
Aiolos transcription factor is a member of the Ikaros family of zinc-finger proteins. Aiolos is a hemopoietic-specific transcription factor that regulates B lymphocyte proliferation and differentiation. It can also participate in chromatin remodeling.
for Aiolos antibody (pAb)Immunogen
This Aiolos antibody was raised against a recombinant GST-tagged protein corresponding to the C-terminal half of mouse Aiolos.
for Aiolos antibody (pAb)Application Notes
This Aiolos antibody is validated for use in Western blot (1:500 to 1:1,000 dilution). The protein migrates around 70 kDa, and multiple isoforms may be detected due to alternative splicing as seen in this example.
for Aiolos antibody (pAb)Buffer
Rabbit antisera with 30% glycerol containing 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Aiolos antibody (pAb).
Aiolos pAb tested by Western blot.
Detection of Aiolos by Western blot analysis. Aiolos was detected using a 1:1,000 dilution of Aiolos pAb with 20 µg mouse spleen tissue extract.
for Aiolos antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Aiolos antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
