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Antibodies

AML-2/Runx3 antibody (pAb)

Aliases: CBFA3, PEBP2aC
Catalog No: 39301 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:44 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

for AML-2/Runx3 antibody (pAb)Application Notes


Validated Applications:
EMSA: 4 - 6 µl per assay

The addition of Tris-glycine electrophoresis buffer for supershift assays is recommended.



Published Applications:
ChIP
WB
ICC
EMSA

See references for more information. Individual optimization may be required.

for AML-2/Runx3 antibody (pAb)Immunogen

This AML-2 antibody was raised against a full-length protein corresponding to human AML-2/Runx3.

for AML-2/Runx3 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for AML-2/Runx3 antibody (pAb)References

 
AML-2/Runx3 antibody (pAb) tested by supershift.

AML-2/Runx3 antibody tested by supershift.
Detection of AML-2/Runx3 by EMSA analysis. AML-2/Runx3 antibody was used in a supershift assay (EMSA) with Raji nuclear extract (lane 4).

for AML-2/Runx3 antibody (pAb)Background

AML-2 (acute myeloid leukemia 2 protein) also known as Runx3 (Runt-related transcription factor 3) is a member of the DNA binding alpha subunit of the heterodimeric transcription factor complex PEBP2/CBF. AML-2 can either activate or repress transcription, and it also interacts with other transcription factors. AML-2 expression is regulated by AML-1/Runx1.

for AML-2/Runx3 antibody (pAb)Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for AML-2/Runx3 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AML-2/Runx3 antibody (pAb).

for AML-2/Runx3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AML-2/Runx3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AML-2/Runx3 antibody (pAb)
 

Data Thumbnails

AML-2/Runx3 antibody (pAb) tested by supershift.

Supershift of AML-2/Runx3 pAb.
(Click image to enlarge and see details.)