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| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| Not recommended. | 6 µl per ChIP | 3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see "Application Notes" below for any recommendations. | ||
for c-Jun antibody (pAb)Background
c-Jun is a component of the AP-1 transcription factor complex in combination with c-Fos. It can be activated by phosphorylation from the JNK pathway.
for c-Jun antibody (pAb)Immunogen
This c-Jun antibody was raised against a synthetic peptide corresponding to amino acid residues 20-37 of human c-Jun.
for c-Jun antibody (pAb)Application Notes
This c-Jun antibody has been validated for use in chromatin IP (3 µl per ChIP) and electrophoretic mobility shift assays (EMSA), and it has been reported to work in immunofluorescence. We recommend adding 6 µl of antibody to the reaction mixture when performing supershift experiments. We also recommend the use of Tris-boric acid electrophoresis buffer. Individual optimization may be required. This antibody is not recommended for Western blotting.
for c-Jun antibody (pAb)Buffer
PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.
for c-Jun antibody (pAb)Positive Control
Active Motif's 3T6 Swiss albino nuclear extract (Catalog No. 36002) can be used as a positive control for c-Jun antibody.
Chromatin IP Kits & Reagents
Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with c-Jun antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with c-Jun antibody (pAb).
c-Jun antibody tested by supershift.
Detection of c-Jun by EMSA analysis. c-Jun antibody was used in a supershift assay (EMSA) with 3T6 Swiss albino nuclear extract (lane 4). Lane 5 demonstrates a supershift competition in the presence of neutralizing peptide.
for c-Jun antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for c-Jun antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
