| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | ChIP, EMSA |
| Reactivity: | Human |
| Molecular Weight: | 53 kDa |
|
| Western Blot |
Chromatin IP |
| Not tested |
5 µl per ChIP |
Background for p53 antibody (pAb):
p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant
p53 that has lost its DNA-binding function interferes with the activity of native
p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for
p53, which causes its destabilization. Inactivation of
p53 is often coincident with hyperactivation of
NFκB (
NFκB p50 and
NFκB p65), both of which serve to inhibit apoptosis.
Chromatin IP Assays:
Active Motif has Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page
Immunogen for p53 antibody (pAb):
This p53 antibody was raised against a synthetic peptide corresponding to amino acid residues 367-381 of human p53.
Application Notes for p53 antibody (pAb):
This p53 antibody has been validated for use in chromatin IP (5 µl per ChIP) and in electrophoretic mobility shift assays (EMSA). Sufficient antibody to perform 17 supershift reactions is provided. We recommend adding 2 µl of antibody to the reaction mixture when performing supershift experiments. We also recommend the use of Tris-glycine electrophoresis buffer. Individual optimization may be required.
Buffer:
PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.
Positive Control:
Active Motif's
Raji nuclear extract (Catalog No. 36023) can be used as a positive control for p53 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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