| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | EMSA |
| Reactivity: | Human, Mouse, Rat |
| Molecular Weight: | 34 kDa |
|
| Western Blot |
Chromatin IP |
| Not recommended |
Not tested |
Background for Pit-1 antibody (pAb):
Pit-1 (
POU1F1,
GHF-1,
growth hormone factor-1,
pituitary specific transcription factor 1) is a
pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the
POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were
Pit-1 and Oct-1 (MIM 164175) of mammals, and Unc-86 of
C. elegans.
Pit-1 activates the production of growth hormone and
Prolactin. In addition,
Pit-1 acts as a
repressor of gene expression, which allows for the differentiation of specific cell types.
Pit-1 is expressed as two alternatively spliced products, designated Pit-1α and Pit-1β, that have molecular masses of 33 and 31 kDa, and differ in their trans-activation ability. Mutations in the
Pit-1 gene is believed to result in
combined pituitary hormone deficiency (
CPHD) for growth hormone, Prolactin and thyroid stimulating hormone.
Immunogen for Pit-1 antibody (pAb):
This antibody was raised against a synthetic peptide corresponding to amino acid residues 107-120 of human Pit-1.
Application Notes for Pit-1 antibody (pAb):
This Pit-1 antibody has been validated for use in electrophoretic mobility shift assays (EMSA). Sufficient reagent to perform 17 supershift reactions is provided. We recommend adding 2 µl of antibody to the reaction mixture when performing supershift experiments. We also recommend the use of Tris-glycine electrophoresis buffer. Individual optimization may be required. This antibody is not recommended for use in Western blotting.
Buffer:
PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.
Positive Control:
Active Motif's
GH3 nuclear extract (Catalog No. 36008) can be used as a positive control for Pit-1 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Pit-1 pAb tested by supershift.
Detection of Pit-1 by EMSA analysis. Pit-1 pAb was used in a supershift assay (EMSA) with GH3 nuclear extract (lane 4). Lane 5 demonstrates a supershift competition in the presence of neutralizing peptide.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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