CHD2 antibody (pAb)

for CHD2 antibody (pAb)Background

CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.

for CHD2 antibody (pAb)Immunogen

This CHD2 antibody (pAb) was raised against a peptide corresponding to the C-terminus of human CHD2.

for CHD2 antibody (pAb)Application Notes

This CHD2 antibody has been validated for use in Western blotting (1:100 - 1:500 dilution). The addition of 0.1% Tween-20 in all blocking solutions may reduce background. Individual optimization may be required.

for CHD2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for CHD2 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CHD2 antibody.

for CHD2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CHD2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.