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Antibodies

IRF-3 antibody (pAb)

Catalog No: 39371 Format: 200 µl $410 Buy Now
Catalog No: 39372 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:50 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated

for IRF-3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:2,000 dilution

Published Applications:
ChIP

for IRF-3 antibody (pAb)Immunogen

This IRF-3 antibody was raised against a peptide corresponding to residues 306-312 of human IRF-3.

for IRF-3 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for IRF-3 antibody (pAb)References

 
IRF-3 antibody (pAb) tested by Western blot.

IRF-3 pAb tested by Western blot.
Detection of IRF-3 by Western blot analysis. The presence of IRF-3 in nuclear extracts derived from U-937 (IFNγ) cells is confirmed using IRF-3 rabbit polyclonal antibody at a 1:1,000 dilution.

for IRF-3 antibody (pAb)Background

IRF-3 (Interferon regulatory factor 3 or IRF3) is a member of the IRF family of transcription factors that include IRF-1, IRF-6 and IRF-7. IRF-3 that has been phosphorylated at serine/threonine forms a complex with CREBBP and translocates to the nucleus to activate transcription of interferon-induced genes.

for IRF-3 antibody (pAb)Positive Control

U-937 nuclear extract (IFN-gamma treated) can be used as a positive control for IRF-3 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with IRF-3 antibody (pAb).

for IRF-3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IRF-3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

IRF-3 antibody (pAb)
 

Data Thumbnails

IRF-3 antibody (pAb) tested by Western blot.

Western blot of IRF-3 pAb.
(Click image to enlarge and see details.)